Fig. 2

(A-D) 3 hpf embryos from crosses of a wild type female and male (A), wild type female with taz^Δ10/Δ10 male (B), taz^Δ10/Δ10 female with wild type male (C) and taz^Δ1/Δ1 female with wild type male (D). Embryos from taz^Δ10/Δ10 or taz^Δ1/Δ1 females are arrested at the 1-cell stage. (E) Embryos from crosses of wild type female and male, wild type female with taz^Δ10/Δ10 male, and taz^Δ10/Δ10 female with wild type male are shown at 1-cell, 2-cell, 4-cell and 8-cell stages. Most embryos produced by taz^Δ10/Δ10 females remain at the 1-cell stage, and a few initiate irregular cleavage planes at wild type 4-cell and 8-cell stage. (F-I) In situ hybridization to detect taz mRNA shows that taz transcripts are found in oocytes and follicle cells in ovary sections (F), and abundant expression in embryos at the 1-cell stage (H) when compared to sense probe control (I). Nonspecific signal is detectable in oocytes at stage I and II while using sense probe as control (G). Oocytes at stages I, II, III are shown; green arrow, the oocyte cortex; green arrowhead, follicle cells. Scale bar, 1 mm in A-D, 0.5 mm in E and 100 μm in F-I.