FIGURE

Fig. 5

ID

ZDB-FIG-190530-7

Publication

Bhakta et al., 2018 - pouC regulates expression of bmp4 during atrioventricular canal formation in zebrafish

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Fig. 5

Precise levels of pouC are required for normal AVC formation. A: Tg(cmlc2:mCherry) zebrafish were injected with control or pouC MO and imaged by fluorescence microscopy at the indicated hpf. A, atrium; V, ventricle; *, pericardial edema. B: Tg(cmlc2‐EGFP) zebrafish embryos were injected with control or pouC MO and imaged by brightfield (top) and fluorescence (bottom) illumination at 24 hpf. Zoomed inset is shown below for both images. C: Titration of injected pouC mRNA into zebrafish showed a dose‐dependent increase in observed cardiovascular phenotypes. D: Zebrafish embryos were injected with control or pouC MO or pouC mRNA, and anf expression was visualized by in situ hybridization. While control embryos showed anf exclusion from the AVC region (black arrowheads), both pouCknockdown and overexpression led to similar anf expression throughout the linear heart tube. E: Zebrafish were injected with control MO, pouC MO, or pouC MO plus 100 pg of pouC mRNA. A, atrium; V, ventricle.

Expression Data

Genes:	GFP mCherry nppa
Fish:	AB AB + MO1-pou6f1 f1Tg zf2186Tg f1Tg + MO1-pou6f1 zf2186Tg + MO1-pou6f1
Knockdown Reagent:	MO1-pou6f1
Anatomical Terms:	atrioventricular canal atrium cardiac muscle cell cardiac ventricle cardiac muscle cell heart cardiac muscle cell heart tube cardiac muscle cell
Stage Range:	Prim-5 to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO1-pou6f1 f1Tg + MO1-pou6f1 zf2186Tg + MO1-pou6f1
Knockdown Reagent:	MO1-pou6f1
Observed In:	atrioventricular canal heart heart morphogenesis
Stage Range:	Prim-5 to Long-pec

Phenotype Detail

Acknowledgments

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