FIGURE

Fig. 8

ID

ZDB-FIG-190528-14

Publication

Blokhina et al., 2019 - The telomere bouquet is a hub where meiotic double-strand breaks, synapsis, and stable homolog juxtaposition are coordinated in the zebrafish, Danio rerio

Other Figures

All Figure Page

Back to All Figure Page

Fig. 8

spo11 -/- males fail to properly synapse homologous chromosomes, and exhibit high numbers of engaged telomeres during meiosis I.

A: 1A-5B: spo11 -/- nuclear surface spreads stained for Sycp3 (green), Sycp1 (blue), telomeres (Tel; magenta), and DNA (DAPI, blue). 1C-5C: Magnified (Mag) images are from regions indicated by white boxes in the panels above. Telomere associations are seen at all stages (arrowheads); Short stretches of Sycp1 are seen in some mutant cells (short arrows). Scale bar = 5μm. B: Numbers of engaged telomeres in WT vs. spo11 -/-cells. The mutant cells were categorized based on similarity to WT Sycp3 loading extent. Post-LZ refers to all cells that were classified as pre-pachytene or pachytene in WT and equivalent cells in spo11 mutants. The data was pooled from two experiments done on different days (set 1, orange circles; set 2, green diamonds). P values were acquired using unpaired t test with Welch’s correction. For * p ≤ 0.0314, and for **** p < 0.0001. While WT telomere engagements are reduced at later stages, the telomere engagements in spo11-/- are maintained. C: Dot plot of BAC distance measurements in WT and spo11 -/- males (in μm). In the WT during the early stages when the average Sycp3 lines are short (< 2 μm), the BAC signals are unpaired; As meiosis progresses and the Sycp3 lines elongate (> 2 μm), the BAC signals exhibit pairing (p = 0.0004, unpaired t test with Welch’s correction). In the spo11 -/- males the BAC signals remain unpaired even with extended Sycp3 axes.

Expression Data

Antibodies:	Ab2-sycp1 Ab2-sycp3
Fish:	spo11^uc73/uc73 (AB)
Anatomical Terms:	testis chromosome testis condensed nuclear chromosome
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	spo11^uc73/uc73 (AB)
Observed In:	chromosome organization involved in meiotic cell cycle meiotic sister chromatid cohesion spermatocyte synaptonemal structure synaptonemal complex organization testis condensed nuclear chromosome
Stage:	Adult

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.