FIGURE

Fig. 6

ID

ZDB-FIG-190528-12

Publication

Blokhina et al., 2019 - The telomere bouquet is a hub where meiotic double-strand breaks, synapsis, and stable homolog juxtaposition are coordinated in the zebrafish, Danio rerio

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Fig. 6

Double strand break (DSB) localization during male zebrafish meiosis I.

A: Testis section stained for γH2AX (green), telomeres (Tel; magenta), and DNA (DAPI, blue). γH2AX staining is polarized when the telomeres are in bouquet formation (triple arrow, white boxes) and is more scattered when the telomeres are dispersed (arrow, orange boxes). Scale bar = 20 μm. B: Panels 1A-4C: Nuclear surface spreads stained for Sycp1 (blue), Rad51 (green), telomeres (Tel, magenta) and DNA (DAPI, blue); 1C-4C: Magnified (Mag) images are from regions indicated by white boxes in the top row panels. White arrowhead points to an example of an Sycp1 stretch with no Rad51 foci. Orange arrowhead points to an example of an Sycp1 stretch with Rad51 foci. 1A-4B scale bar = 5μm.

Expression Data

Antibodies:	Ab1-h2afx Ab2-rad51 Ab2-sycp1
Fish:	AB NHGRI-1
Anatomical Terms:	testis site of double-strand break testis synaptonemal complex
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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