Fig. 6

Exposure to constant light prevents leads to small apical domains in maturing PRCs. (A) IS length and OS volume of LWS cones of fish kept at different light conditions are shown: constant light (yellow), light/dark cycle (grey) and constant dark (black). All measurements are represented in dot plots with calculate minimum, mean, and maximum. On average, 65 cells from 4–6 independent embryos were quantified per timepoint. Statistical significance was calculated by a one-way ANOVA followed by Tukey's multiple comparison test. The significance is as follows: ns (non-significant) =P>0.05, *=P≤0.05, **=P≤ 0.01, ****=P≤0.0001. (B) Confocal images of retinal sections of Tg(LWS) zebrafish embryos at 120 hpf. Embryos were exposed to different light conditions, constant dark, light/dark cycle (14 h/10 h) and constant light. mKate2-tagged opsin is shown in red and phalloidin staining in grey. Outer segments are highlighted by arrowheads. Scale bars: 5 µm.