FIGURE

Fig. 5

ID

ZDB-FIG-181130-28

Publication

Castillo-Robles et al., 2018 - smarce1 mutants have a defective endocardium and an increased expression of cardiac transcription factors in zebrafish

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Fig. 5

Defects in the endocardium in smarce1 mutants. Embryos at 9- (A,B), 14- (C,D) and 20- (E,F) somite stage showing the expression the endothelial marker flk1. While a normal ALPM pattern is observed in mutant embryos up to 14 somites, at 20 somite stage mutant embryos present an abnormal distribution of endocardial cells. Expression of nfatc1 in embryos at 26 (G-I), 30 (J–L) and 35 hpf (M,N) reveals a disorganized pattern of the endocardium. Embryos at 24 hpf showing expression of the endocardial marker gata5 (O–Q) and the myocardial marker cmlc2 (T–V). Expression of gata5 in the endocardium of the mutant embryos is abnormal and has a lower signal. A dysmorphic pattern for cmlc2 is detected in one out of six embryos (V). R,S. Expression of nppa in 48 hpf embryos. An arrow shows the absence of nppa at the atrioventricular junction. (W, X) Expression of versican in embryos at 4 dpf. Scale bars: 50 μm.

Expression Data

Genes:	gata5 kdrl myl7 nfatc1 nppa vcana
Fish:	WT smarce1^una1/una1
Anatomical Terms:	anterior lateral plate mesoderm anterior lateral plate mesoderm endothelial cell atrial endocardium atrial myocardium atrioventricular canal atrium cardiac ventricle ventricular endocardium ventricular myocardium
Stage Range:	5-9 somites to Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	smarce1^una1/una1
Observed In:	atrioventricular canal atrioventricular canal morphogenesis atrium cardiac ventricle endocardium endothelial cell endocardium formation endocardium morphogenesis
Stage Range:	20-25 somites to Day 4

Phenotype Detail

Acknowledgments

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