FIGURE

Fig. 4

ID

ZDB-FIG-181116-5

Publication

Zhang et al., 2018 - Hrg1 promotes heme-iron recycling during hemolysis in the zebrafish kidney

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Fig. 4

Defects of heme-iron recycling in kidney macrophages of Hrg1 DKO zebrafish.

(A) qRT-PCR of hrg1a and hrg1b to quantify mRNA expression in the kidney, spleen and liver from control (non-PHZ treated) and 1-day post PHZ-treatment samples. Three adult zebrafish were pooled as one cohort, and three cohorts were repeated as biological triplicates. * p<0.05. (B) IHC staining of Hrg1 proteins in sections from the kidney, spleen and liver of adult zebrafish. (C) DAB-enhanced perl’s iron staining of kidney sections from Tü and hrg1 DKO zebrafish with control (non-PHZ), 1 day, 2 days and 3 days post PHZ-treatment. (D) O-dianisidine staining of kidney sections from Tü and hrg1 DKO zebrafish with control (non-PHZ), 1 day, 2 days and 3 days post PHZ-treatment. Scale bar: 20μm.

Expression Data

Genes:	slc48a1a slc48a1b
Antibody:	Ab1-slc48a1
Fish:	TU
Condition:	chemical treatment by environment: phenylhydrazine
Anatomical Terms:	kidney kidney macrophage liver spleen
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	TU slc48a1a^{zf2122/zf2122}; slc48a1b^{zf2121/zf2121}
Condition:	chemical treatment by environment: phenylhydrazine
Observed In:	kidney kidney heme metabolic process kidney iron molecular entity spleen
Stage:	Adult

Phenotype Detail

Acknowledgments

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