IMAGE

Fig. 4

ID

ZDB-IMAGE-181116-5

Genes

Antibodies

Ab1-slc48a1

Source

Figures for Zhang et al., 2018

Image

Figure Caption

Fig. 4

Defects of heme-iron recycling in kidney macrophages of Hrg1 DKO zebrafish.

(A) qRT-PCR of hrg1a and hrg1b to quantify mRNA expression in the kidney, spleen and liver from control (non-PHZ treated) and 1-day post PHZ-treatment samples. Three adult zebrafish were pooled as one cohort, and three cohorts were repeated as biological triplicates. * p<0.05. (B) IHC staining of Hrg1 proteins in sections from the kidney, spleen and liver of adult zebrafish. (C) DAB-enhanced perl’s iron staining of kidney sections from Tü and hrg1 DKO zebrafish with control (non-PHZ), 1 day, 2 days and 3 days post PHZ-treatment. (D) O-dianisidine staining of kidney sections from Tü and hrg1 DKO zebrafish with control (non-PHZ), 1 day, 2 days and 3 days post PHZ-treatment. Scale bar: 20μm.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

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