FIGURE

Fig. 3

ID

ZDB-FIG-181024-21

Publication

Swaminathan et al., 2018 - Non-canonical mTOR-Independent Role of DEPDC5 in Regulating GABAergic Network Development

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Fig. 3

depdc5 Knockout Alters Gene Expression in Larval Brains without Affecting Brain Morphology

(A) Volcano plot showing the differentially expressed gene expression profile between depdc5^+/+ and depdc5^−/− larval brains. See also Table S1.

(B) Pathways showing high enrichment in the differentially expressed genes (upregulated are shown in green; downregulated are in red).

(C) Whole-mount images of depdc5^+/+ and ^−/− larvae at different stages of development immunostained with α-acetylated tubulin (n > 3/genotype/developmental stage). Scale bars, 40 μm.

(D) Measurement of head size and head surface area in 3-dpf depdc5^+/+ and depdc5^−/− larvae.

(E) Whole-mount images of 48-hpf depdc5^+/+ and ^−/− larvae immunostained with α-phosphorylated histone H3 (n > 3/genotype).

(F) Cresyl violet staining comparing 7-dpf depdc5^+/+ (top half) and depdc5^−/− (bottom half) larval heads showed no gross difference in the density of cells in the brain. Scale bar, 100 μm.

Expression Data

Antibody:	Ab1-tuba
Fish:	TL depdc5^{udm102/udm102} (TL)
Anatomical Term:	brain axon
Stage Range:	Prim-5 to Days 7-13

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	depdc5^{udm102/udm102} (TL)
Observed In:	brain brain axon development brain mitotic cell cycle brain neurogenesis head
Stage Range:	Prim-5 to Days 7-13

Phenotype Detail

Acknowledgments

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