FIGURE

Fig. 1

ID
ZDB-FIG-181024-19
Publication
Swaminathan et al., 2018 - Non-canonical mTOR-Independent Role of DEPDC5 in Regulating GABAergic Network Development
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Fig. 1

depdc5 Is Essential for Survival

(A) Whole-mount in situ hybridization showing expression of depdc5 at different stages of development.

(B) Electropherograms showing confirmation of the 4-bp insertion by Sanger sequencing.

(C) Expression of depdc5 transcript in 7-dpf depdc5+/+, +/−, and −/− larvae. N = 3; Student’s t test: p(WT versus heterozygote [HT]) = 0.0026; p(WT versus homozygote [HM]) = 0.001.

(D) Survival curve showing that depdc5−/− embryos have reduced survival, with most larvae dying around 14 dpf (N = 2, n = 15 per genotype).

(E) Images showing that depdc5−/− larvae do not show gross morphological changes at 3 dpf.

(F) Measurement of body length (top; n = 20/genotype; p = 0.0045 by unpaired Student’s t test) and eye size (bottom; n = 12/genotype; p = 0.7219 by unpaired Student’s t test) showed no significant change in either parameter.

Expression Data
Gene:
Fish:
Anatomical Terms:
Stage Range: 14-19 somites to Days 7-13

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Observed In:
Stage Range: Protruding-mouth to Adult

Phenotype Detail
Acknowledgments
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