FIGURE

Fig. 2

ID

ZDB-FIG-181009-8

Publication

Zhang et al., 2018 - Distant Insulin Signaling Regulates Vertebrate Pigmentation through the Sheddase Bace2

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Fig. 2

Bace2 Acts during Melanophore Differentiation

(A) At 24 hpf, ISH shows the bace2^−/− mutants have unaffected expression for neural crest markers crestin and sox10.

(B) But at 72 hpf, ISH shows mRNA for the pigmentation genes dct and pmela are elevated in bace2^−/− mutants, especially in the head (arrowhead).

(C and D) Consistently, bace2^−/− mutants have increased numbers of pigmented melanophores in the head (arrowhead). Fish number n(WT) = 55, n(bace2^−/−) = 30, two-tailed t test, ^∗∗p < 0.01.

(E) Treatment of WT embryos with 100 μM Bace2 inhibitor (PF-06663195) from 48 to 72 hpf is sufficient to phenocopy bace2^−/−. Data are from two independent experiments, with total fish numbers: n(DMSO) = 21, n(shield-24h) = 45, n(shield-48h) = 56, n(shield-72h) = 72, n(24h-48h) = 58, n(24h-72h) = 40, n(48h-72h) = 49; one-way ANOVA followed by Holm-Sidak's multiple comparisons test, ^∗∗p < 0.01. All bar graphs are presented as means ± SEM. Scale bars, 200 μm.

See also Figures S1 and S2.

Expression Data

Genes:	crestin dct pmela sox10
Fish:	AB bace2^{hu3332/hu3332} (AB)
Anatomical Terms:	head neural crest pigment cell
Stage Range:	Prim-5 to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	bace2^{hu3332/hu3332} (AB)
Observed In:	head head melanocyte pigment cell
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Cell, 45(5), Zhang, Y.M., Zimmer, M.A., Guardia, T., Callahan, S.J., Mondal, C., Di Martino, J., Takagi, T., Fennell, M., Garippa, R., Campbell, N.R., Bravo-Cordero, J.J., White, R.M., Distant Insulin Signaling Regulates Vertebrate Pigmentation through the Sheddase Bace2, 580-594.e7, Copyright (2018) with permission from Elsevier. Full text @ Dev. Cell