FIGURE

Fig. 1

ID

ZDB-FIG-181004-15

Publication

Bickers et al., 2018 - Zebrafish snai2 mutants fail to phenocopy morphant phenotypes

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Fig. 1

Snai2 endogenous expression in wild-type embryos.

Expression of snai2 was analyzed via whole mount in situ hybridization at 14 hpf (A), 18 hpf (B), and 24 hpf (C). Embryos were cryosectioned post-in situ at 18 hpf (D) and 24 hpf (F). Simplified schematics are provided (E and G). Double fluorescent in situ for snai2 with endothelial markers fli1a at 14 hpf (H) and etsrp at 26 hpf (I) was performed. Insets show a close-up view of the PLM. QPCR was used to compare snai2 enrichment within double positive HSPCs sorted from Tg(CD41:GFP/kdrl:mCherry) on 2 dpf to the rest of the embryo. Markers cmyb and kdrl, which should be enriched in this population, are displayed alongside for comparison. N: notochord; NT: neural tube; S: somite.

Expression Data

Genes:	etsrp fli1 snai2
Fish:	AB la2Tg; s896Tg
Anatomical Terms:	endothelial cell mesoderm nervous system somite somite ventral region
Stage Range:	10-13 somites to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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