Conservation of Myosin I protein function. a, b Loss of myo1d results in increased nuclear fragmentation. Tg(dusp6:GFP-MA) or myo1d MZ; Tg(dusp6:GFP-MA) embryos injected with H2BmCherry mRNA exhibit fragmented nuclei in KV epithelial cells and quantified as fragmented nuclei per KV sample. Fragmented nuclei are represented as absolute numbers per KV Z-section. c?f Overexpression of amoeba myo1C (n?=?13) or rat Myo1d (n?=?15) were sufficient to rescue lumen area in myo1d MZ embryos compared to uninjected controls (n?=?16) (d), circularity (n?=?16 myo1d MZ, n?=?10 rat Myo1d injected MZ embryos) (e), and reduced vacuole size (n?=?9 myo1d MZ, n?=?10 rat Myo1d injected MZ embryos) (f) in myo1d MZ mutants. However, rat Myo1d lacking the TH1 domain (myo1d?TH1) (n?=?12) failed to restore lumen size (d). Unpaired t test or one-way ANOVA with post hoc Turkey?s multiple range tests were conducted with, **p?<?0.01, ***p?<?0.005, ****p?<?0.0001 represents statistical significance. ns not significant. Data shown are mean?±?SEM
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