Conservation of Myosin I protein function. a, b Loss of myo1d results in increased nuclear fragmentation. Tg(dusp6:GFP-MA) or myo1d MZ; Tg(dusp6:GFP-MA) embryos injected with H2BmCherry mRNA exhibit fragmented nuclei in KV epithelial cells and quantified as fragmented nuclei per KV sample. Fragmented nuclei are represented as absolute numbers per KV Z-section. c–f Overexpression of amoeba myo1C (n = 13) or rat Myo1d (n = 15) were sufficient to rescue lumen area in myo1d MZ embryos compared to uninjected controls (n = 16) (d), circularity (n = 16 myo1d MZ, n = 10 rat Myo1d injected MZ embryos) (e), and reduced vacuole size (n = 9 myo1d MZ, n = 10 rat Myo1d injected MZ embryos) (f) in myo1d MZ mutants. However, rat Myo1d lacking the TH1 domain (myo1d∆TH1) (n = 12) failed to restore lumen size (d). Unpaired t test or one-way ANOVA with post hoc Turkey’s multiple range tests were conducted with, **p < 0.01, ***p < 0.005, ****p < 0.0001 represents statistical significance. ns not significant. Data shown are mean ± SEM
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