Fig. 6

P300/CBP histone acetyltransferase activity inhibition induces muscle cells detachment in zebrafish. (a–c) Confocal projections of transgenic Tg(smyhc1:EGFP)ⁱ¹⁰⁴ embryos treated with either DMSO (vehicle), 2 μM or 3.5 μM C646 at 10 hpf for 24 h, fixed and imaged at 6 dpf. (a’–c’): higher magnification views of the boxed area in a–c. Arrowheads indicate muscle cell attachment abnormalities. (d–g) Confocal projections of transgenic Tg(smyhc1:EGFP)ⁱ¹⁰⁴ embryos treated with either DMSO or 2 μM C646 from 10 hpf for 24 h, and further raised in water (−Methylcellulose; d,e) or in viscous medium (+Methylcellulose; f,g) until 5 dpf. (h–m) Immunostaining against either H3K27ac (h–j) or p53ac (k–m). Confocal sections of embryos treated with either DMSO (vehicle), or 2 μM or 3.5 μM C646 at 10 hpf for 10 h and fixed at 20 hpf, as indicated on the panels. Fluorescent intensity is color-coded. (n) Relative mRNA expressions were determined by RT-qPCR in 3 dpf embryos treated with either DMSO, or 2 μM or 3.5 μM C646 from 10 hpf for 24 h. Error bars represent S.D. Statistical significance is relative to control and was calculated with an unpaired t-test. ns: not significant; *p < 0.05, **p < 0.01, ***p < 0.001. A minimum of 12 embryos were analysed per condition.