FIGURE

Fig. 7

ID

ZDB-FIG-180917-30

Publication

Svahn et al., 2018 - Nucleo-cytoplasmic transport of TDP-43 studied in real time: impaired microglia function leads to axonal spreading of TDP-43 in degenerating motor neurons

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Fig. 7

Axonal redistribution of eGFP-TDP43^WT during UV-induced degeneration and microglia depletion. a Pre-irradiation, line is a projection of the axon vector used for signal analysis. Scale = 5 µm. bi–iv At each time point from left to right the three windows represents a single time point illustrating the cell body and the TDP-43 distribution (soma, eGFP-TDP43^WT in green), the axonal projection (axon, mTagBFP in magenta incl. neurite vector as yellow line), and the fluorescence intensity of eGFP in the segments along the neurite vector. c Line graph demonstrating the time course of the mean eGFP fluorescence intensity along the axon post-stress induction. Scale = 2 µm. Note: axonal redistribution was observed in 2 of the 10 neurons, which were targeted for UV injury and underwent degeneration

Expression Data

Genes:	EGFP TagBFP
Fish:	mq10Tg; mq14Tg mq10Tg; mq14Tg + MO1-spi1b
Condition:	light damage: primary motor neuron
Knockdown Reagent:	MO1-spi1b
Anatomical Terms:	primary motor neuron axon primary motor neuron cytoplasm primary motor neuron nucleus
Stage Range:	Protruding-mouth to Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	mq10Tg; mq14Tg + MO1-spi1b
Condition:	light damage: primary motor neuron
Knockdown Reagent:	MO1-spi1b
Observed In:	microglial cell primary motor neuron primary motor neuron axon primary motor neuron cytoplasm
Stage Range:	Protruding-mouth to Day 5

Phenotype Detail

Acknowledgments

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