Fig. S8

ES treatment improves morphological defects observed in Coa6 knockdown embryos. (A) Zebrafish embryos were treated with indicated concentrations of ES and (B) copper, and combination of both at 3 hours post fertilization (hpf). The surviving fish were counted at the indicated time points. (n ≥30 for each treatment). (C) Table describing phenotype scores (1-4) at different time points in zebrafish embryo development. (D) Phenotypic scores of untreated and ES treated Coa6 knockdown zebrafish embryos at the indicated time points (hpf, hours post fertilization). Batches of 50 fertilized embryos injected with either translation blocking or mismatch control morpholinos were treated with or without 100 nM ES at 3 hpf, before making observations at the indicated time points. (n≥30 for each time point and treatment). (E) Representative images of untreated and ES treated Coa6 knockdown zebrafish embryos at 72 and 96 hpf. The arrow heads indicates cardiac edema. (F) Heart rate of zebrafish embryos treated with either mismatch control morpholino or the Coa6 translation blocking morpholino were measured at the indicated time point. (n≥30 per group; data represent mean ± SEM).