Fig. S4

Hsa.CTGF-based Yap1/Taz zebrafish reporter is modulated by glucocorticoids and blood flow. (A,A’) Representative image of Tg(Hsa.CTGF:nlsmCherry)^ia49 transgenic embryos injected with Yap1/Taz or control morpholinos alone or combined with 25 μM Dexamethasone (DEX) from 24 hpf to 48 hpf. Dex-activation of Hsa.CTGF transgene is maintained in control morpholino-injected embryos and ablated in Yap/Taz morphants. (A’’) Average values of fluorescence integrated density calculated for treated embryos and controls. For each group a minimum of 10 embryos were analyzed. (B) Representative confocal images of Tg(Hsa.CTGF:nlsmCherry)^ia49/tg(kdrl:GFP) double transgenic embryos injected with control or sih morpholinos at 48 hpf. In sih morphants, the activity of the hCTGF reporter in the endothelium is reduced. (B’) Average values of fluorescence integrated density calculated for treated embryos (n=5) and controls (n=6). The mCherry fluorescence was measured in the endothelial cells and normalized for the GFP fluorescence used as internal standard. A.U., arbitrary units. ** = p<0.01.