FIGURE

Fig. 4

ID
ZDB-FIG-180823-8
Publication
Moreno et al., 2018 - Investigation of Islet2a function in zebrafish embryos: Mutants and morphants differ in morphologic phenotypes and gene expression
Other Figures
All Figure Page
Back to All Figure Page
Fig. 4

Islet2a morphant but not mutant embryos displayed altered CaP morphology.

(A-E) In live tg(mnx1:gfp) 24 hpf embryos, CaP neurons expressed gfp in their somas and axons. (A, B) Injection of a T-MO (MO) led to truncation of ventrally projecting axons (asterisks, B) compared to control (Ctl, A), as previously reported [15, 17]. (C-E) In wildtype (wt, C), heterozygous (het, D) and mutant (mut, E) islet2a embryos, CaP neuron axon growth and trajectories appeared normal regardless of genotype. Sample size ranged from 8–30 per condition. Scale bar in E, for A-E: 50 μm. (F, G) In fixed non-transgenic 28 hpf embryos, zn1/znp1 immunoreactivity did not reveal any differences in CaP axon morphology between wildtype (F; n = 9) versus mutant (G; n = 9) embryos.

Expression Data
Gene:
Antibodies:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Prim-5

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Knockdown Reagent:
Observed In:
Stage: Prim-5

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One