FIGURE

Fig. 8

ID

ZDB-FIG-180820-9

Publication

Zhong et al., 2018 - Ezh2 promotes clock function and hematopoiesis independent of histone methyltransferase activity in zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. 8

Definitive hematopoiesis is disrupted in ezh2–/– mutant zebrafish. (A–L) In situ hybridization of WT sibling (A, C, E, G, I and K) and ezh2–/– mutant embryos or larvae (B, D, F, G, J and L) using probes of runx1 (A, B), cmyb (C, D), ikaros (E, H), lck (I, J), rag-1 (K, L) at 28 hpf (A, F) and 96 hpf (G, L), respectively. White arrowheads indicate ICM in A, C and E and black arrowheads the thymus in G, I and K. Scale bar, 0.25 mm. (M–P) Images of cmyb:eGFP cells in wild-type (M, O) and ezh2–/– mutant (N, P) fish at 32 hpf (M, N) and 72 hpf (O, P). (Q–T) Images of coro1a:eGFP cells in wild-type (Q, S) and ezh2–/– mutant (R, T) fish at 28 hpf (Q, R) and 72 hpf (S, T). White arrowheads indicate ICM in M, N, Q and R and dashed circle the thymus in O, P, S and T. Scale bar in (M–T), 0.25 mm. All images shown are lateral view, anterior to left.

Expression Data

Genes:	EGFP ikzf1 lck myb rag1 runx1
Fish:	WT ezh2^{sa1199/sa1199} hkz04tTg zf169Tg ezh2^{sa1199/sa1199}; hkz04tTg ezh2^{sa1199/sa1199}; zf169Tg
Anatomical Terms:	intermediate cell mass of mesoderm thymus ventral wall of dorsal aorta
Stage Range:	Prim-5 to Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ezh2^{sa1199/sa1199} ezh2^{sa1199/sa1199}; hkz04tTg ezh2^{sa1199/sa1199}; zf169Tg
Observed In:	definitive hemopoiesis hematopoietic stem cell differentiation intermediate cell mass of mesoderm thymus ventral wall of dorsal aorta
Stage Range:	Prim-5 to Day 4

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nucleic Acids Res.