Fig. S8

myosin1 and vangl2 genes control Left-Right Organizer flow a, WT embryos display a higher flow velocity in the anterior compared to the posterior KV (n=28). This antero-posterior asymmetry is reduced in MZ myo1D (n=63) and vangl2 (n=36) mutants, but restored in MZ myo1D ; vangl2+/- (n=25). MZ myo1D ; MZ myo1G double mutants (n=29) maintain a higher flow speed in the anterior KV, suggesting that their LR asymmetry defects are mainly due to the observed overall reduction in KV flow velocity and circularity (Fig.4h and c below). The antero-posterior velocity gradient is diminished in WT embryos injected with myo1Cb RNA (n=37), but restored again upon removal of one copy of vangl2 (n=19). b-e, Temporal projections of trajectories of fluorescent microspheres in the KV lumen. Compared to WT, MZ myo1D ; MZ myo1G double mutants or WT embryos injected with myo1Cb RNA display altered KV flows. Flow pattern is partially restored when myo1Cb RNA is injected in embryos heterozygous for vangl2. Dorsal views of 8 somites stage KVs, anterior up. Quantifi-cations and display items are from the same dataset that is also used in Fig. 4a-h. Scale bar: 20 μm.