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Genetic inactivation of zebrafish myo1D a, Sequence chromatograms and schematic representation of myo1D mutants generated by Crispr/Cas9 mutagenesis. b, myo1D homozygous mutant adult fish are indistinguishable from heterozygous or homozygous wild-type siblings. c, Whole-mount in situ hybridization reveals that myo1D transcripts are maternally supplied and can be detected at the 8-cell stage (animal pole view), well before the activation of the zygotic genome. d, e, Zygotic myo1D transcripts are present at low levels throughout the embryo. At segmentation stages, increased transcript levels are detected in the notochord. d, dorsal view, anterior up. e, lateral view, anterior to the left. Scale bars: 100 μm.
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