Fig. 7
- ID
- ZDB-FIG-180730-26
- Publication
- Eno et al., 2018 - Modulation of F-actin dynamics by maternal mid1ip1L controls germ plasm aggregation and furrow recruitment in the zebrafish embryo
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F-actin cortical trenches and furrow indentations depend on aura/mi1ip1L function. (A-J) Orthogonal views of cortical and furrow F-actin in wild-type and aura mutant embryos treated with drugs targeting F-actin. Wild-type embryos exhibit cortical trenches (A) and furrow indentations (B), which are missing in aura mutants (G,H). In wild type, actin inhibition with cytochalasin D abolishes the formation of both cortical trenches (C) and furrow indentations (D). Treatment with the F-actin stabilizer phalloidin allows cortex trenches (E) and furrow indentations (F) in wild type and rescues the formation of these structures in aura mutants (I,J). (K,L) Quantitation of the number of cortical trenches and furrow indentations under the various conditions, using data from at least seven images per condition. Significance was determined using an unpaired t-test. Data are mean±s.e.m. Scale bar: 10 μm. |