FIGURE

Fig. 5

ID

ZDB-FIG-180730-24

Publication

Eno et al., 2018 - Modulation of F-actin dynamics by maternal mid1ip1L controls germ plasm aggregation and furrow recruitment in the zebrafish embryo

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Fig. 5

Modulation of germ plasm RNP outward movement depends on cortical F-actin. (A-H) RNPs (green) and cortical F-actin (red) in control- (DMSO), cytochalasin D-, phalloidin- and nocodazole-treated wild-type (A-D) and aura mutant (E-H) embryos. F-actin inhibition with cytochalasin D in wild-type embryos leads to large RNP aggregates at the distal edge of the blastodisc (B), mimicking the aura mutant phenotype (E; large spherical structures are retained cortical granules; Eno et al., 2016). F-actin stabilization with phalloidin (C,G) does not have a significant effect in wild type (C) but reduces ectopic RNP aggregate accumulation in aura mutants (G). Inhibition of microtubules with nocodazole (D,H) results in reduced outward RNP movement in both wild type (D; Theusch et al., 2006) and aura mutants (H). Brackets indicate ectopic marginal RNP aggregate accumulation. (I,J) Quantitation of RNP outward clearing. Significance was determined using an unpaired t-test. Data are mean±s.e.m. Scale bar: 10 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB mid1ip1l^t9792
Conditions:	chemical treatment by environment: cytochalasin D chemical treatment by environment: nocodazole chemical treatment by environment: phalloidin
Observed In:	actin filament organization blastodisc peripheral region blastomere ribonucleoprotein complex
Stage Range:	1-cell to 8-cell

Phenotype Detail

Acknowledgments

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