Fig. S11
Knock-down of tbx2b reduces the ectopic opn1sw1 expression in bsx mutant embryos. (A-D) Dorsal view on embryos 2 dpf after in situ hybridization using opn1sw1 probe. (A) Wildtype embryos injected with standard control morpholino do not express opn1sw1 in the pineal gland, and neither do (C) embryos after tbx2b morpholino injection. (B) Ectopic pineal gland expression of opn1sw1 in bsx mutant embryos is (D) significantly decreased after injection of tbx2b morpholino. (E) Measurements of integrated staining intensities (see Fig.S10) of opn1sw1 in the pineal complex reveal that the effect of tbx2b morpholino knockdown is significant (Wilcoxon-Mann-Whitney test). (A-D) Anterior is to the top. Scale bar: 50 µm. |