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Bmp but not Wnt signaling regulates bsx expression in the PC. (A-D,I-L′) Transgenic embryos (as indicated in boxes) and wild-type control siblings were heat-shocked for 1 h from 12 to 13 hpf and fixed either at 16 hpf (A-D) or 24 hpf (I-L′). (E-H) Embryos were treated with the Wnt inhibitor IWR-1 from 2 hpf onwards and fixed at 24 hpf. bsx or flh expression were detected by WISH as indicated. (A-H,I′,J′,K′,L′) Dorsal views, orientations as indicated in (A); (I,J,K,L) lateral views, orientations as indicated in (J). Numbers for representative phenotype shown and embryos analyzed: (A) 19/19, (B) 20/20, (C) 14/14, (D) 17/17, (E) 18/18, (F) 14/14, (G) 19/19, (H) 13/15, (I,I′) 26/26, (J,J′) 27/27, (K,K′) 16/16 and (L,L′) 15/16. Scale bars: 100 µm.
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