Fig. 2

Midzone Microtubule Assembly and YCL Microtubule Dynamics Defects in MZdchs1b

(A) Representative time-lapse still images of microtubule dynamics during cytokinesis in WT and MZdchs1b embryos using Tg[ef1α:dclk-GFP]. Arrowheads denote the initial midzone microtubule width. Scale bar, 30 μm.

(B) Quantification of the midzone microtubule assembly activities in WT and MZdchs1b. The Y axis shows the ratio of midzone microtubule width d at each time point divided by the initial width d’. N, number of embryos. ^∗∗∗∗p < 0.0001, paired Student's two-tailed t test was used to determine the difference between control and mutants across the time course. Error bars represent SEM.

(C) Representative still time-lapse images of YCL microtubules marked with Tg[ef1α:dclk-GFP] during cleavage stages in WT and MZdchs1b. To the right a cartoon of eight-celled embryo with the black box illustrating the analyzed region; A, animal pole; V, vegetal pole. Scale bar, 30 μm.

(D) Quantification of YCL microtubule density during individual cell cycles from the time-lapse videos in WT and MZdchs1b. N, number of embryos. Error bars represent SEM.

(E) Quantification of EB3-GFP track speed in the YCL in WT and MZdchs1b. The Y axis shows the relative frequency of all quantified tracks in corresponding speed bins. The right panel shows a representative speed heatmap in the region of interest. A, animal pole; V, vegetal pole. N, number of embryos; n, number of EB3 tracks.

(F) Directional distribution of EB3-GFP track angles in WT and MZdchs1b. N, number of embryos; n, number of EB3 tracks.

See also Figure S6, Videos S3 and S4.