Fig. 1

Disrupted Embryonic Cleavages in MZdchs1b

(A) Representative time-lapse still images of cleavage furrow calcium signaling indicated by Tg[βactin2:GCaMP6s]^stl351 during cleavage stages in WT and MZdchs1b. Scale bar, 300 μm.

(B) Quantification of cleavage furrow orientation by monitoring calcium signaling in WT and MZdchs1b at 2–4 cell stage. Directional distributions of the positioning angle θ is shown as a rose diagram of 5° bins. Each bin represents a percentage of the total population with the circular line indicating the percentile. N, number of embryos. p < 0.005, Mardia-Watson-Wheeler test.

(C) Quantification of calcium signaling dynamics during furrow propagation in WT and MZdchs1b at 2–4 cell stage. The Y axis shows the percentage of furrow calcium signaling width d at each time point divided by the total cell width d’. N, number of embryos.

(D) Quantification of the ratio of the first furrow deepening calcium signaling width at the onset of second cleavage in WT and MZdchs1b. The Y axis shows the ratio of the initial cleavage calcium signaling width w divided by the total cell width w’. N, number of embryos. ^∗∗∗p < 0.001, Student's unpaired t test. Error bars represent SD.

(E) Representative images of mitotic and cytokinesis events in the blastomeres stained using γ-tubulin (green) antibody and counterstained with DAPI (blue). Scale bar, 30 μm. Arrowheads indicate two or more nuclei in one cell, asterisk indicates abnormal chromatin bridge, and arrow indicates micronuclei.

(F) Quantification of mitotic events in WT and MZdchs1b embryos at 2.5 hpf. N, number of embryos. n, number of cells.

See also Figure S1, Video S1.