FIGURE

Fig. 2

ID

ZDB-FIG-180713-9

Publication

Zhang et al., 2018 - The DEAD box RNA helicase Ddx39ab is essential for myocyte and lens development in zebrafish

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Fig. 2

Loss of ddx39ab leads to cardiomyocyte differentiation defects in zebrafish embryos. (A) Ventral view of heart from whole-mount wild-type or ddx39ab mutant zebrafish embryos at 36 hpf, with cranial to the top. Myocardium was labeled with MF20 antibody. Scale bars: 200 μm. (B) RNA in situ hybridization and qPCR results for cardiogenic regulatory gene expression in wild-type and ddx39ab mutant zebrafish embryos at 26 hpf. (C) RNA in situ hybridization and qPCR results for cardiomyocyte structural gene expression in wild-type and ddx39ab mutant zebrafish embryos at 26 hpf. (B,C) Frontal views with dorsal side to the top. At least 15 (A) or 20 (B,C) embryos for each genotype were analyzed and representative samples are shown. For qPCR results, data are mean±s.e.m. n.s, not significant. *P<0.05, **P<0.01, ***P<0.001.

Expression Data

Genes:	acta1b bmp4 cmlc1 gata5 myh6 myh7 nkx2.5 nppa tbx5a ttn.2
Antibodies:	Ab-MF20 Ab2-tnnt2
Fish:	WT ddx39ab^{nju203Gt/nju203Gt}
Anatomical Terms:	heart tube heart tube sarcomere myocardium whole organism
Stage Range:	Prim-5 to Prim-25

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ddx39ab^{nju203Gt/nju203Gt}
Observed In:	cardiac chamber formation heart heart looping heart tube heart tube sarcomere whole organism
Stage Range:	Prim-5 to Prim-25

Phenotype Detail

Acknowledgments

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