FIGURE

Fig. 1

ID

ZDB-FIG-180711-65

Publication

Frøyset et al., 2018 - Astroglial DJ-1 over-expression up-regulates proteins involved in redox regulation and is neuroprotective in vivo

Other Figures

All Figure Page

Back to All Figure Page

Fig. 1

Generation of zebrafish with astroglial over-expression of DJ-1. A, Transgenic zebrafish expressing Flag-tagged DJ-1, Tg(gfap:egfp-2A-flag-zDJ-1), were generated using ISce1-transgenesis. Flag-tagged DJ-1 expression in astrocytes was controlled by Glial Fibrillary Acidic Protein (GFAP) promotor elements. A viral 2A peptide allows expression of GFP and Flag-DJ1 as uncoupled proteins from the same transgene. B, Western blot shows expression of Flag-DJ1 from total protein extracts of larvae 24–120 hpf. Ponceau S staining is used as loading control. C, GFP expression localized astroglia, neural retina and spinal cord was observed from 48 hpf. c1–3, Immunoblots of brain sections (4 dpf) showing astroglial GFP and neuronal tyrosine hydroxylase expression. Tyrosine hydroxylase was used as a marker for dopaminergic cells.

Expression Data

Gene:	EGFP
Antibody:	Ab3-th
Fish:	ub1001Tg
Anatomical Terms:	astrocyte dopaminergic neuron retina spinal cord
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Redox Biol.