FIGURE SUMMARY
Title

Astroglial DJ-1 over-expression up-regulates proteins involved in redox regulation and is neuroprotective in vivo

Authors
Frøyset, A.K., Edson, A.J., Gharbi, N., Khan, E.A., Dondorp, D., Bai, Q., Tiraboschi, E., Suster, M.L., Connolly, J.B., Burton, E.A., Fladmark, K.E.
Source
Full text @ Redox Biol.
Fig. 1

Generation of zebrafish with astroglial over-expression of DJ-1. A, Transgenic zebrafish expressing Flag-tagged DJ-1, Tg(gfap:egfp-2A-flag-zDJ-1), were generated using ISce1-transgenesis. Flag-tagged DJ-1 expression in astrocytes was controlled by Glial Fibrillary Acidic Protein (GFAP) promotor elements. A viral 2A peptide allows expression of GFP and Flag-DJ1 as uncoupled proteins from the same transgene. B, Western blot shows expression of Flag-DJ1 from total protein extracts of larvae 24–120 hpf. Ponceau S staining is used as loading control. C, GFP expression localized astroglia, neural retina and spinal cord was observed from 48 hpf. c1–3, Immunoblots of brain sections (4 dpf) showing astroglial GFP and neuronal tyrosine hydroxylase expression. Tyrosine hydroxylase was used as a marker for dopaminergic cells.

EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Anatomical Terms:
Stage: Long-pec
Fig. 2

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Fig. 3

ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

Fig. 4

ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Redox Biol.
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