FIGURE

Fig. 4

ID

ZDB-FIG-180706-59

Publication

Pfeiffer et al., 2018 - Rapid progression through the cell cycle ensures efficient migration of primordial germ cells - the role of Hsp90

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Fig. 4

Hsp90aa1.2 function is important for microtubule organization. (A) In control PGCs, the single MTOC is positioned at the back of the migrating cell (n = 31 cells, 4 cells showing two MTOCs (13%); upper panels), while in embryos in which Hsp90aa1.2 is knocked down, a large proportion of the cells show a duplicated MTOC (n = 22 cells, 11 cells with two MTOCs (50%); lower panels). (B) In embryos mutated for hsp90aa1.2, duplicated MTOCs are found in the migrating PGCs (n = 41 cells, 22 cells with two MTOCs (52%); lower panels), a phenomenon reversed by introduction of RNA encoding for Hsp90aa1.2 in PGCs by fusing the open reading frame of the RNA to the 3’UTR of the nanos3 gene (n = 51 cells, 9 cells with two MTOCs (18%); upper panels). The experiments were performed using the hsp90aa1.2d34 and the hsp90aa1.2d5 alleles, as well as in embryos harboring both alleles.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	hsp90aa1.2^mu9/mu9 (AB) hsp90aa1.2^mu10/mu10 (AB) AB + MO1-hsp90aa1.1,hsp90aa1.2
Knockdown Reagent:	MO1-hsp90aa1.1,hsp90aa1.2
Observed In:	primordial germ cell primordial germ cell microtubule organizing center primordial germ cell microtubule organizing center organization
Stage Range:	Shield to 75%-epiboly

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 436(2), Pfeiffer, J., Tarbashevich, K., Bandemer, J., Palm, T., Raz, E., Rapid progression through the cell cycle ensures efficient migration of primordial germ cells - the role of Hsp90, 84-93, Copyright (2018) with permission from Elsevier. Full text @ Dev. Biol.