FIGURE

Fig. S7

ID

ZDB-FIG-180705-66

Publication

Williams et al., 2018 - Gon4l regulates notochord boundary formation and cell polarity underlying axis extension by repressing adhesion genes

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Fig. S7

Reduction of EpCAM does not suppress cell polarity defects in MZudu-/- gastrulae

a-b) Live images of otic vesicles in WT embryos at 30hpf either (a) uninjected or (b) injected with 1ng epcam MO. Arrowheads mark otoliths (or lack thereof). Approximately 36% of epcam MO-injected embryos from three independent trials lacked otoliths as shown in b, an additional 56% exhibited smaller otoliths (both phenotypes are observed in epcam mutants²). c-f) Quantification of axial mesoderm cell orientation (e) and elongation (f) in epcam overexpressing WT gastrulae. Asterisks indicate significant differences compared to WT controls (Kolmogorov-Smirnov (e) and Mann-Whitney (f) tests, **p<0.01, ***p<0.001, ****p<0.0001). Black bars are median values in e, mean values in f; medians and means of WT are shown as gray bars in e-f. g-n) Quantification of axial mesoderm cell orientation and elongation in control MZudu-/- siblings (g-j) and MZudu-/- injected with 1ng epcam MO (k-n) at the time points indicated. Graphs and color coding as in Fig. 3. Bars are median values in i, m; bars are mean values in j, n. N indicates the number of embryos analyzed, scale bar is 50μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.