Med12 is epistatic to Apc and is required for Wnt-dependent gene expression. A-D are all lateral views of 48 hpf embryos. (A,A′) wild-type embryos. (B) apchu745/hu745 mutant. (C,C′) med12y82/y82 mutants. (D,D′) apc hu745/hu745; med12y82/y82 double mutant. In A′,C′ and D′, the black dotted line represents the distance from the yolk to the tail tip and the red dotted line represents the tail length. (E) Tail curvature of apc hu745/hu745; med12y82/y82 double mutants was compared to that of wild-type and med12y82/y82 mutant embryos. Tail curvature is the tail length divided by the distance from yolk to tail tip. wild type: n=20, med12y82/y82: n=24, apc hu745/hu745; med12y82/y82: n=8. A, P<0.001; B, P<0.05, two-tailed unpaired Student's t-test. Error bars show s.e.m. (F) The number of melanophores on the lateral surface of the yolk of apc hu745/hu745; med12y82/y82 double mutants was compared to that of wild-type and med12y82/y82 mutant embryos. wild type: n=15, med12y82/y82: n=27, apc hu745/hu745; med12y82/y82: n=9. A,B P<0.001, two-tailed unpaired Student's t-test. Error bars show s.e.m. (G,G′) Representative images of GFP expression visualized at ×100 magnification in 24 hpf wild-type siblings and med12 mutants that are heterozygous for the TOP:GFP transgene. Arrows indicate area of reduced GFP expression. (H) Corrected head fluorescence was compared between wild-type and med12y82/y82 mutant embryos. Corrected head fluorescence is head fluorescence (Integrated Density) divided by the area of the head. wild type: n=8, med12y82/y82: n=9. P<0.001, two-tailed unpaired Student's t-test. Error bars show s.e.m. (I,I′) Representative full embryo views of GFP expression magnified ×50 in 24 hpf wild-type siblings and med12 mutants that are heterozygous for the TOP:GFP transgene. Dashed line indicates an example of the area (the head) that was circled for fluorescence and area measurements in H. J,J′ are dorsal views of WISH for lef1 in 48 hpf embryos magnified ×100. (J) wild-type sibling. (J′) med12 mutant.
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