FIGURE

Fig. S9

ID

ZDB-FIG-180622-40

Publication

Missinato et al., 2018 - Dusp6 attenuates Ras/MAPK signaling to limit zebrafish heart regeneration

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Fig. S9

BCI215 is specific for Dusp6 (A) Cryosections of WT and dusp6^pt30a/+ hearts at 7 dpa injected for 6 days with DMSO (WT n=33; dusp6^pt30a/+ n=17), or 0.25 mg/Kg of BCI215 (WT n=7; dusp6^pt30a/+ n=11), stained for Mef2c and PCNA. (B) Quantification of cardiomyocyte proliferation in hearts injected with BCI215, or vehicle DMSO. Sub-optimal dose of BCI215 does not affect cardiomyocyte proliferation in WT hearts, but increases cardiomyocyte proliferation in dusp6^pt30a/+ hearts. **p<0.01; n.s.=not significant. One-way ANOVA. (C) Cryosections of WT and dusp6^pt30a/+ hearts at 7 dpa injected with DMSO (WT n=25; dusp6^pt30a/+ n=15), or 0.5 mg/Kg of BCI215 (WT n=21; dusp6^pt30a/+ n=8), stained for Mef2c and PCNA. (D) Quantification of cardiomyocyte proliferation. Optimal dose of BCI215 increases cardiomyocyte proliferation in WT hearts but it does not affect cardiomyocyte proliferation in dusp6^pt30a/+ hearts ****p<0.0001; n.s.=not significant. One-way ANOVA. Scale bars, 100 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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