FIGURE

Fig. S4

ID

ZDB-FIG-180622-23

Publication

Di Donato et al., 2018 - An Attractive Reelin Gradient Establishes Synaptic Lamination in the Vertebrate Visual System

Other Figures

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Fig. S4

Reelin immunoreactivity in the neuropil of 3 dpf reln^-/- larvae. Related to Figure 4.

(A) Immunostaining of anti-reelin (green) and DAPI (blue) on a horizontal cryosection of a 3 dpf larval tectum. Scale bar = 20μm. The yellow rectangle indicates the long-axis along which the Reelin gradient in (B) was measured.

(B) Densitometric plot of normalized anti-reelin fluorescence intensity taken from 3 different tecta at 3dpf. Data are represented as mean ± s.e.m.

(C, D) Horizontal sections of 5dpf tectum showing reelin spatial distribution detected wildtype larva (C) or a reln^-/- mutant (D). The reelin protein is absent in the mutant. Scale bars = 30 μm.

Expression Data

Gene:	reln
Antibody:	Ab1-reln
Fish:	WT reln^{ic3032/ic3032}
Anatomical Term:	tectal neuropile
Stage Range:	Protruding-mouth to Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	reln^{ic3032/ic3032}
Observed In:	tectal neuropile
Stage:	Day 5

Phenotype Detail

Acknowledgments

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