FIGURE

Fig. 5

ID

ZDB-FIG-180427-26

Publication

Hui et al., 2017 - Zebrafish Regulatory T Cells Mediate Organ-Specific Regenerative Programs

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Fig. 5

zT_reg Cells Promote Regenerative Precursor Cell Proliferation

(A and B) zT_reg cells (arrows) interacting with proliferating neural progenitor cells (A; arrowheads point to Sox2⁺PCNA⁺) and newly differentiated neurons (B; arrowheads point to HuC/D⁺EdU⁺). Right panels show single-channel confocal sections of the demarcated regions. The dotted line marks the border of the central canal (cc).

(C) zT_reg cells (arrows) interacting with proliferating cardiomyocytes (arrowheads point to MHC⁺EdU⁺). Image on the right shows the xz and yz planes of the demarcated region.

(D) zT_reg cells (arrows) interacting with proliferating Müller glia (arrowheads point to gfap:GFP⁺PCNA⁺). Right panels show single-channel confocal sections of the demarcated region.

(E–H) Proliferation of neural progenitor cells (E), newly differentiated neurons (F), cardiomyocytes (G), and Müller glia (H). Insets show single-channel confocal slices (E, F, and H) or epifluorescent images (G) of the demarcated regions. Arrows mark co-labeled cells. Dotted lines indicate the wound border. epi, injury epicenter.

(I–L) Quantification of (E), (F), (G), and (H), respectively (mean ± SEM, n = 6–7).

Confocal projections of z stacks are shown, except for the epifluorescent images in (G). ^∗∗p < 0.01, Mann-Whitney U test. Scale bars, 50 μm.

Expression Data

Genes:	EGFP GFP TagRFP
Antibodies:	Ab-F59 Ab1-elavl Ab2-pcna Ab3-sox2
Fish:	vcc3Tg/+ vcc10Tg/+ mi2001Tg/+; vcc3Tg/+
Conditions:	cold damage: heart perforation: retina transection: spinal cord
Anatomical Terms:	regenerating tissue cardiac muscle cell regenerating tissue heart regenerating tissue Muller cell regenerating tissue neuroblast regenerating tissue neuron regenerating tissue retina regenerating tissue spinal cord regenerating tissue T cell
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	vcc5Tg/+ mi2001Tg/+; vcc5Tg/+
Conditions:	chemical ablation: T cell, chemical treatment by environment: metronidazole, cold damage: heart chemical ablation: T cell, chemical treatment by environment: metronidazole, perforation: retina chemical ablation: T cell, chemical treatment by environment: metronidazole, transection: spinal cord
Observed In:	Muller cell cell population proliferation regenerating tissue cardiac muscle cell proliferation regenerating tissue cell population proliferation regenerating tissue neural precursor cell proliferation regenerating tissue neuroblast proliferation
Stage:	Adult

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Cell, 43, Hui, S.P., Sheng, D.Z., Sugimoto, K., Gonzalez-Rajal, A., Nakagawa, S., Hesselson, D., Kikuchi, K., Zebrafish Regulatory T Cells Mediate Organ-Specific Regenerative Programs, 659-672.e5, Copyright (2017) with permission from Elsevier. Full text @ Dev. Cell