FIGURE

Fig. 8

ID

ZDB-FIG-180426-12

Publication

McGurk et al., 2017 - In vivo zebrafish morphogenesis shows Cyp26b1 promotes tendon condensation and musculoskeletal patterning in the embryonic jaw

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Fig. 8

Cyp26b1 function before 60 hpf is necessary for mandibulohyoid junction formation. (A,D) Embryos raised in media containing 0.01% DMSO develop normally. (B) 100% of embryos treated with talarozole between 54 and 60 hpf displayed ectopic muscle projection in the second pharyngeal arch, and the intermandibularis posterior muscles overextended in 58.3% of embryos (n = 64). (C) Among embryos treated from 60 to 72 hpf, 76.9% had mild ectopic muscle projections, but 96.2% developed normal tendon and end-to-end muscle connections at the mandibulohyoid junction (n = 26). (E,F) Talarozole-treated embryos recapitulated cyp26b1 mutant skeletal phenotypes of Meckel’s cartilage fusion (red arrowheads and insets), ceratohyal fusion (black arrowheads and insets), and basihyal reduction (arrows). All images ventral view, anterior to the left. Scale bar = 50 μm

Expression Data

Genes:	EGFP mCherry
Fish:	fb301Tg; pc11Tg
Condition:	chemical treatment by environment: talarozole
Anatomical Terms:	hyoid muscle hyoid muscle tendon cell mandibular muscle mandibular muscle tendon cell
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	fb301Tg; pc11Tg
Condition:	chemical treatment by environment: talarozole
Observed In:	basihyal cartilage anterior region ceratohyal cartilage medial region embryonic viscerocranium morphogenesis Meckel's cartilage medial region sternohyoid skeletal muscle cell ventral intermandibularis posterior muscle tissue morphogenesis ventral intermandibularis posterior skeletal muscle cell
Stage:	Day 4

Phenotype Detail

Acknowledgments

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