FIGURE

Fig. 7

ID

ZDB-FIG-180418-15

Publication

Lu et al., 2017 - Twa1/Gid8 is a β-catenin nuclear retention factor in Wnt signaling and colorectal tumorigenesis

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Fig. 7

Zebrafish Twa1 is essential for dorsoventral patterning during embryogenesis. Zebrafish embryos at the one-cell stage injected with the indicated morpholinos targeting Twa1a and Twa1b (Twa1a-MO and Twa1b-MO) or mRNAs were harvested at the different times, and subjected to the following analyses. (A, B) Microscopy showed the different types of embryos at 24 h post-fertilization (hpf). The classes 1-3 represent increasing degrees of phenotypic severity in eye and brain structures. The percentages of embryos with the different classes are shown. (C-F) Whole-mount in situ hybridization of the dorsal (gsc, chordin and otx2) and ventral (gata2 and eve1) markers and the Wnt target gene boz. The percentages of embryos with abnormal expression of the indicated markers are also presented. β-Cat2, β-catenin 2; boz, bozozok; n, number of observed embryos; Twa1a^*, zebrafish Twa1a-MO-resistant mRNA; Twa1a-ΔCRA^*, zebrafish Twa1a-MO-resistant mRNA without CRA domain; Twa1b^*, zebrafish Twa1b-MO-resistant mRNA.

Expression Data

Genes:	chrd dharma eve1 gata2a gsc otx2b
Fish:	AB AB + MO1-gid8b AB + MO3-ctnnb2
Knockdown Reagents:	MO1-gid8b MO3-ctnnb2
Anatomical Terms:	anatomical structure whole organism dorsal region whole organism ventral region
Stage Range:	Oblong to Bud

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO1-gid8a AB + MO1-gid8b AB + MO3-ctnnb2
Knockdown Reagents:	MO1-gid8a MO1-gid8b MO3-ctnnb2
Observed In:	anatomical structure whole organism whole organism dorsal region whole organism ventral region
Stage Range:	Oblong to Prim-5

Phenotype Detail

Acknowledgments

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