FIGURE

Fig. 6

ID

ZDB-FIG-180327-8

Publication

Goodman et al., 2017 - Integrin α8 and Pcdh15 act as a complex to regulate cilia biogenesis in sensory cells

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Fig. 6

The Itga8–Pcdh15a complex regulates endocytosis/recycling in hair cells. (A–F,H–M) Confocal images of 3 dpf (A–F,H–J) and 5 dpf (K–M) larvae (labeled as in Fig. 1) incubated with FM1-43 for 30 min. Scale bars: 5.5 µm. (G,N) Quantification of total fluorescent intensity per neuromast. At least five independent experiments were performed for each treatment and total fluorescence intensity expressed as a percentage of that in control (mean±s.e.m.). (O) Cartoon of a neuromast (top view) showing the stained structures: hair cells loaded with FM1-43 are in red, and the hair cell bundle in green. **P<0.01; ***P<0.001; ns, not significant (one-way ANOVA followed by Dunnett's multiple comparisons test or two-tailed Student's t-test).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	pcdh15a^{tc256e/tc256e} pcdh15a^{th263b/th263b} WT + MO1-itga8 + MO2-itga8 WT + MO1-pcdh15a
Knockdown Reagents:	MO1-itga8 MO1-pcdh15a MO2-itga8
Observed In:	neuromast hair cell endocytosis
Stage Range:	Protruding-mouth to Day 5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Cell Sci.