FIGURE

Fig. 5

ID

ZDB-FIG-180321-16

Publication

Campbell et al., 2017 - Phosphodiesterase Inhibitors Sildenafil and Vardenafil Reduce Zebrafish Rod Photoreceptor Outer Segment Shedding

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Fig. 5

Rod outer segment shedding resumes following removal of sildenafil. (A) Schematic timeline for examining rod outer segment renewal in larval Tg(Xla.rho:EGFP); Tg(hsp70:HA-mCherryTM); albb4/b4 fish following heat shock at 5 dpf, sildenafil treatment for 3 days post heat shock (dpHS), and 1 to 3 days sildenafil removal (dWash). (B) Representative images of photoreceptor layers from 0 and 3 dWash sildenafil-treated larvae immunolabeled with anti-GFP (green), anti-HA (red), and anti-Rhodopsin (blue) antibodies. Images are projections of a subset of z-sections totaling 8.1 μm. Scale bar: 10 μm. Quantification of shedding distance (DS) following removal of (C) sildenafil for 0 to 3 days (dWash). Lower and upper hinges of box correspond to first and third quartiles; middle corresponds to median; whiskers extend 1.5 * interquartile range above and below the hinges; dots represent outliers. Graphs represent n = 5 retinas/condition (≥74 outer segments). *95% CI of difference compared to control does not span zero.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Invest. Ophthalmol. Vis. Sci.