FIGURE

Fig. 6

ID

ZDB-FIG-180321-17

Publication

Campbell et al., 2017 - Phosphodiesterase Inhibitors Sildenafil and Vardenafil Reduce Zebrafish Rod Photoreceptor Outer Segment Shedding

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Fig. 6

Cone photoreceptors are sensitive to sildenafil and vardenafil. (A) Schematic timeline for examining green cone outer segment length in 8 dpf Tg(SWS1:mCherry); albb4/b4 larvae after bathing in phosphodiesterase inhibitor for 3 days (dTreat). (B) Representative images of photoreceptor layers from larvae treated with 0.05% DMSO, sildenafil, and vardenafil at indicated concentrations. Retinal sections were immunolabeled with green cone opsin antibody (green); ultraviolet cones express mCherry (red). Images are projections of a subset of z-sections totaling 7.33 μm. Scale bar: 10 μm. (C) Quantification of green cone outer segment full length (Dtotal). Lower and upper hinges of box correspond to first and third quartiles; middle corresponds to median; whiskers extend 1.5 * interquartile range above and below the hinges; dots represent outliers. Graph represents n = 5 fish/condition (DMSO = 162 outer segments, sildenafil = 169 outer segments, and vardenafil ≥ 175 outer segments). *95% CI of difference compared to control does not span zero.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Invest. Ophthalmol. Vis. Sci.