FIGURE

Fig. s2

ID

ZDB-FIG-180308-12

Publication

Zhang et al., 2017 - Endoderm Jagged induces liver and pancreas duct lineage in zebrafish

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Fig. s2

Supplementary Figure 2. Canonical Notch activity in liver and pancreas. (a-c)

Immunofluorescence analysis showing all Nkx6.1+ cells in the pancreas (P) are Tp1:GFP+ (green, Notch active) (a). (b-c) Notch active, Tp1:GFP+ cells (blue) endothelial cells expressing flk1:mCherry (red) can also be found associated with the pancreas. These endothelial cells (magenta, yellow arrowheads) do not expression Prox1 (gray), Nkx6.1 (green, duct, b), or Islet1 (green, endocrine, c). However, a subset of Islet1+ endocrine cells of the pancreas can be Tp1:GFP+ (c, white arrowheads). (d) Prox1+ (red) cells in the liver that are Tp1:GFP+ (green) are always observed to not express Hnf4a (blue), indicative of intrahepatic duct cells (white arrowheads). Bottom panels with TP1:GFP (a, d) or DAPI (b,c) channels removed. Scale bars 100μM (a), and 25μM (b-d)

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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