FIGURE

Fig. 3 S1

ID

ZDB-FIG-180207-19

Publication

LeBert et al., 2018 - Damage-induced reactive oxygen species regulate vimentin and dynamic collagen-based projections to mediate wound repair.

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Fig. 3 S1

Vimentin depletion reduced vimentin expression but did not affect fin development.

(A) RT-PCR of morpholino knockdown of vimentin with morpholino 1 and 2 (also see Materials and methods). (B) Morpholino knockdown of vimentin reduced regenerate length. (C-E) The defect in regenerate length and area was observed with either MO1 or MO2. (F-H) Morpholino knockdown of vimentin did not alter caudal fin area or length in unwounded larvae at 5 dpf. Regenerate area measurements for control morpholino and vim MO1 in D were also included in Figure 3B and length measurements for control morpholino and vim MO1 in E were also included in Figure 3—figure supplement 1B Data in G and H represent a single experiment performed in duplicate. For all other graphs, data was pooled from 2 or 4 replicate experiments. ***p<0.001, ****p<0.0001.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO1-vim AB + MO2-vim
Condition:	resection: caudal fin
Knockdown Reagents:	MO1-vim MO2-vim
Observed In:	caudal fin fin regeneration regenerating fin
Stage:	Day 5

Phenotype Detail

Acknowledgments

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