FIGURE

Fig. 7

ID

ZDB-FIG-180131-17

Publication

Escobar-Aguirre et al., 2017 - Microtubule-actin crosslinking factor 1 (Macf1) domain function in Balbiani body dissociation and nuclear positioning

Other Figures

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Fig. 7

Characterization of macf1a^p1CH1, macf1a^p2CH1CH2 and macf1a^p3PRD mutants.

DiOC₆ (mitochondria, green) and Buc (red) staining in early and late stage I oocytes. macf1a^p1CH1 /macf1a^sa12708 and macf1a^p2CH1CH2 /macf1^sa12708 ovaries display a macf1a null phenotype, whereas macf1a^p3PRD / macf1^sa12708 show no phenotype. macf1a^p1CH1 ovaries showed incomplete penetrance, displaying either a WT or a macf1a null phenotype. macf1a^p2CH1CH2 oocytes displayed a macf1a null phenotype, and macf1a^p3PRD showed no phenotype. DiOC₆ and Buc; N≥ 3 ovaries of each genotype, 31 macf1a^p1CH1, 25 macf1a^p1CH1/macf1a^sa12708, 11 macf1a^p2CH1CH2/ macf1a^sa12708, 22 macf1a^p2CH1CH2, 15 macf1a^p3PRD/macf1^sa12708 and 24 macf1a^p3PRD oocytes. DAPI (blue) stains the DNA. Dotted white lines outline the nucleus. Images are single optical sections. Arrowheads in indicate the Bb, and in B the egg cytoplasm. Scale bar: 20 μm.

Expression Data

Antibody:	Ab1-buc
Fish:	TU macf1a^p1CH1/p1CH1 macf1a^{p2CH1CH2/p2CH1CH2} macf1a^p3PRD/p3PRD macf1a^sa12708/+; macf1a^p1CH1/+ macf1a^sa12708/+; macf1a^p2CH1CH2/+ macf1a^sa12708/+; macf1a^p3PRD/+
Anatomical Terms:	oocyte stage I cell cortex oocyte stage I mitochondrial cloud
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	macf1a^p1CH1/p1CH1 macf1a^{p2CH1CH2/p2CH1CH2} macf1a^p3PRD/p3PRD macf1a^sa12708/+; macf1a^p1CH1/+ macf1a^sa12708/+; macf1a^p2CH1CH2/+ macf1a^sa12708/+; macf1a^p3PRD/+
Observed In:	oocyte stage I cell cortex oocyte stage I mitochondrial cloud oocyte stage I nucleus oocyte stage I organelle disassembly
Stage:	Adult

Phenotype Detail

Acknowledgments

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