FIGURE

Fig. 3

ID

ZDB-FIG-180131-14

Publication

Escobar-Aguirre et al., 2017 - Microtubule-actin crosslinking factor 1 (Macf1) domain function in Balbiani body dissociation and nuclear positioning

Other Figures

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Fig. 3

Localization of Macf1a in stage I oocytes.

A-C) Macf1a immunostaining (green) in stage I oocytes shows localization nuclearly and within the Bb (A), and later at the cortex during Bb disassembly (B, C). D) Macf1a staining was negative in a macf1a^sa12708 mutant oocyte. E-G) GFP staining in Gt(macf1a–citrine)^ct68a line also showed Macf1a-Citrine localization to the Bb and at the cortex during disassembly. DAPI (blue/magenta) stains DNA. A-D) N≥ 3 ovaries, 20 isolated WT oocytes were examined (see methods), 16 oocytes showed Macf1 nuclear staining; 18 macf1a^sa12708 oocytes were examined. E-H) N = 3 ovaries, 9 of 20 Gt(macf1a–citrine)^ct68a/+ oocytes showed nuclear staining (H). Representative images from 3 experiments. Dotted white lines outline the nucleus. Images are a sum of 3 single optical confocal sections. Arrowheads indicate the Bb. Scale bars: 20 μm.

Expression Data

Genes:	Citrine macf1a
Fish:	TU macf1a^{sa12708/sa12708} macf1a^ct68aGt
Anatomical Terms:	oocyte stage I cell cortex oocyte stage I mitochondrial cloud oocyte stage I nucleus
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	macf1a^{sa12708/sa12708}
Observed In:	oocyte stage I
Stage:	Adult

Phenotype Detail

Acknowledgments

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