FIGURE

Fig. S3

ID

ZDB-FIG-180126-25

Publication

Matsuoka et al., 2017 - CNS-resident progenitors direct the vascularization of neighboring tissues

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Fig. S3

Characterization of VTAs and the PNVP. (A–A″) 8 dpf Tg(kdrl:EGFP);Tg(-0.8flt1:tdTomato) trunk vasculature. Vessels that exhibit strong Tg(-0.8flt1:tdTomato) expression comprise those of arterial identity, and VTAs also show strong Tg(-0.8flt1:tdTomato) expression (white arrows). (B–B″) 8 dpf Tg(kdrl:EGFP);Tg(lyve1:DsRed) trunk vasculature. Vessels that show Tg(lyve1:DsRed) expression comprise those of venous and lymphatic identity, and VTAs are not positive for Tg(lyve1:DsRed) expression (white arrows). (C) Percentage of VTAs that show strong Tg(-0.8flt1:tdTomato) expression at 8 dpf (132 VTAs quantified in 12 fish). All of the VTAs examined at 8 dpf show strong Tg(-0.8flt1:tdTomato) expression. (D and E) 11 dpf Tg(kdrl:EGFP) larval trunk vasculature. Sprouting vessels in the dorsal trunk emerge from ISVs or VTAs (yellow arrows). (F) High-magnification image of Tg(kdrl:EGFP) trunk vasculature at 15 dpf. Vessels that emerge around the spinal cord are connected to the VTAs (yellow arrows). (Scale bar, 100 µm.)

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA