FIGURE

Fig. 1

ID

ZDB-FIG-180118-39

Publication

Zabinyakov et al., 2017 - Characterization of the first knock-out aldh7a1 zebrafish model for pyridoxine-dependent epilepsy using CRISPR-Cas9 technology

Other Figures

All Figure Page

Back to All Figure Page

Fig. 1

QIAxcel system, Sanger sequencing and HRM analysis results. A) Gel image produced by QIAxcel system revealing wild type aldh7a1 sequence, as well as the heterozygous and homozygous 5bp deletion. The DNA sequences shown in the lower panel reveal the complement reverse sequences. The broken line indicates the nucleotides that were deleted by CRISPR/Cas9 in the homozygous fish. B) The HRM melting plots are displayed. The heterozygous sequence is identified by the double peak. The homozygous sequence is identified by a peak at 76.5 degrees Celsius, and the wild type sequence is identified by a peak at 77.5 degrees Celsius.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One