FIGURE

Fig. 5

ID

ZDB-FIG-180116-17

Publication

Quintana et al., 2017 - Functional analysis of the zebrafish ortholog of HMGCS1 reveals independent functions for cholesterol and isoprenoids in craniofacial development

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Fig. 5

Inhibition of isoprenoids and cholesterol has not effect on sonic hedgehog (Shh) activity.

(A-C) Whole mount in situ hybridization (ISH) was performed at 3 days post fertilization (protruding mouth stage) (dpf) with embryos treated with 0.01% DMSO (vehicle), 8uM lonafarnib (Lona) or 2.5 uM Ro 48 8071 (Ro-48). (DMSO n = 10, Ro-48 n = 13, and Lona n = 16). (D) Sybr green based quantitative real time PCR was performed on hmgcs1 mutant embryos (Homozygous MT) or their wildtype siblings (WT Sibling) at the indicated days post fertilization (DPF) to detect mRNA expression of gli1. Error bars represent standard deviation of technical replicates.

Expression Data

Gene:	ptch2
Fish:	AB
Conditions:	chemical treatment by environment: lonafarnib chemical treatment by environment: Ro 48-8071
Anatomical Terms:	brain ventral region oral ectoderm pharyngeal pouch
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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