Fig. 2

hmgcs1 does not regulate neural crest cell specification or migration.

(A&B) In situ hybridization to detect sox10 expression was performed in hmgcs1 homozygous mutants or siblings at the 18 somite stage. Dorsal view with anterior to the left. (n = 15 including 8 wildtype/heterozygous siblings and 7 homozygous mutants). Wildtype Sibling is depicted in A, but no significant differences were observed between wildtype and heterozygous carriers. Arrowheads indicated two positive streams of Sox10+ positive cells. (C&D) Sagittal view of wildtype hmgcs1 siblings (hmgcs1 Sibling) or hmgcs1 homozygous mutant larvae (hmgcs1 MT). Larvae were maintained in a Tg(sox10:tagRFP) background and RFP was visualized using traditional microscopy at 1 day post fertilization (dpf) between Prim-5 and Prim-15. n = 20 including 15 wildtype and heterozygous individuals and 5 homozygous mutant individuals. Sibling depicted is a wildtype individual. No significant difference between wildtype embryos was detected.