FIGURE

Fig. 3

ID

ZDB-FIG-180112-22

Publication

Hsu et al., 2017 - Overexpression of microRNA-722 fine-tunes neutrophilic inflammation by inhibiting Rac2 in zebrafish.

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Fig. 3

Overexpression of rac2 rescues neutrophil recruitment in miR-722-expressing larvae. (A) Tg(lyzC:miR-722/Dendra2)^pu6 (miR-722) was crossed with Tg(mpx:mCherry-2A-Rac2) (Rac2) and the offspring were separated into four groups. For control, Tg(lyzC:miR-722/Dendra2)^pu6 was crossed with Tg(mpx:mCherry) (mCherry). All experiments were performed with F2 larvae at 3 dpf. (B) Tracks and quantification of neutrophil motility in indicated lines. Results were pooled from three independent larvae. P values were calculated with unpaired Student's t-test. Scale bar: 100 µm. (C,D) Quantification of neutrophil recruitment to tail wounding (C) or localized ear infection (D) in siblings separated into the four groups as depicted in A. One representative experiment of three independent biological repeats is shown (n=20 for each group). P values were calculated with unpaired two-way ANOVA.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	pu6Tg; zf306Tg (AB)
Conditions:	bacterial treatment by injection: Pseudomonas aeruginosa PAK amputation: post-vent region
Observed In:	neutrophil otic vesicle neutrophil post-vent region neutrophil
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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